Hotspot

petN-psbM

LSC · 30221-31580 · 1360 bp

Back to Byrsonima

Pi 0.00303

MarkerSeek score 61.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1360
variable_sites: 4
pi: 0.00303
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGGGGAAGAAGTGGACTCT	TGCACTGTTCATTCTAGTCCC	50.0	47.6	57.6	57.7	1434-1455 bp	1.0	68.1
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1440-1461 bp	1.0	67.5
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	GCACTGTTCATTCTAGTCCCT	50.0	47.6	57.6	57.4	1433-1454 bp	1.0	65.9
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TCATTCTAGTCCCTACTGCCT	50.0	47.6	57.6	57.3	1426-1447 bp	1.0	65.3
petN-psbM_p5	CTCGCTTGGGCTGCTTTAAT	TGCACTGTTCATTCTAGTCCC	50.0	47.6	58.9	57.7	1487-1508 bp	1.0	63.7