Hotspot

atpH-atpI

LSC · 14813-15908 · 1096 bp

Back to Byrsonima

Pi 0.00274

MarkerSeek score 60.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1096
variable_sites: 3
pi: 0.00274
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1183-1186 bp	1.0	78.8
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	1175-1178 bp	1.0	78.6
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	GCGAATCCATAGAAGGGCAT	45.0	50.0	58.0	57.8	1154-1157 bp	1.0	78.4
atpH-atpI_p4	AGCGGCAGAAATCAATGGAT	GCGAATCCATAGAAGGGCAT	45.0	50.0	57.9	57.8	1146-1149 bp	1.0	78.2
atpH-atpI_p5	GTCCAATAGAAGCAAGCCCA	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1221-1224 bp	1.0	78.1