Hotspot

atpA-atpF

LSC · 12704-12761 · 58 bp

Back to Byrsonima

Pi 0.069

MarkerSeek score 83.60

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 58
variable_sites: 4
pi: 0.069
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CAATGCCGTCACCTACTTGA	TGTTTGGGTCGATGCAAGAA	50.0	45.0	57.9	58.0	208 bp	1.0	80.6
atpA-atpF_p2	TACTAATCTCGTCGGCTCGA	TGTTTGGGTCGATGCAAGAA	50.0	45.0	57.8	58.0	121 bp	1.0	80.6
atpA-atpF_p3	CAATGCCGTCACCTACTTGA	ATGTTTGGGTCGATGCAAGA	50.0	45.0	57.9	57.8	209 bp	1.0	79.2
atpA-atpF_p4	CAATGCCGTCACCTACTTGA	CATGTTTGGGTCGATGCAAG	50.0	50.0	57.9	57.7	210 bp	1.0	78.6
atpA-atpF_p5	ACTTGAAGTACGGTACCGGT	TGTTTGGGTCGATGCAAGAA	50.0	45.0	58.4	58.0	194 bp	1.0	78.4