Hotspot

rps16-trnQ

LSC · 5997-7546 · 1550 bp

Back to Byrsonima

Pi 0.00518

MarkerSeek score 61.90

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 1550
variable_sites: 8
pi: 0.00518
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1636-1656 bp	1.0	79.8
rps16-trnQ_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1620-1640 bp	1.0	78.9
rps16-trnQ_p3	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1631-1651 bp	1.0	78.4
rps16-trnQ_p4	AGCCGATCATGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	1637-1657 bp	1.0	77.4
rps16-trnQ_p5	CCGAGAGCAACTTCATTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	1692-1712 bp	1.0	76.9