Hotspot

matK-trnK-UUU

LSC · 3633-4348 · 716 bp

Back to Byrsonima

Pi 0.00559

MarkerSeek score 62.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 4
pi: 0.00559
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACCATGATCATGAGCAAGTGT	TGAGGGTTGCTAACTCAACG	42.9	50.0	57.6	57.8	858-864 bp	1.0	68.7
matK-trnK-UUU_p2	ACCATGATCATGAGCAAGTGT	AACGGTAGAGTACTCGGCTT	42.9	50.0	57.6	58.2	842-848 bp	1.0	68.7
matK-trnK-UUU_p3	ACCATGATCATGAGCAAGTGT	ACGGTAGAGTACTCGGCTTT	42.9	50.0	57.6	58.2	841-847 bp	1.0	68.7
matK-trnK-UUU_p4	ACTCCCGAAAGATAAGTGGG	TGAGGGTTGCTAACTCAACG	50.0	50.0	56.6	57.8	829-835 bp	1.0	68.7
matK-trnK-UUU_p5	CTCCCGAAAGATAAGTGGGT	TGAGGGTTGCTAACTCAACG	50.0	50.0	56.6	57.8	828-834 bp	1.0	68.7