Hotspot

ycf1

SSC · 126334-131946 · 5613 bp

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Pi 0.111
MarkerSeek score 75.40
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5613
variable_sites
1199
pi
0.111
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GGTTTGTGTAGTCGGTCCAA ACATACCTGCCGAGTTTGTC 50.0 50.0 57.8 57.9 968 bp 0.25 47.3
ycf1_p2 GGTTTGTGTAGTCGGTCCAA CCCTCACGCAGAAAGATCAA 50.0 50.0 57.8 57.9 698 bp 0.25 47.3
ycf1_p3 AAAGGGGTTTGTGTAGTCGG ACATACCTGCCGAGTTTGTC 50.0 50.0 57.7 57.9 973 bp 0.25 47.1
ycf1_p4 AAAGGGGTTTGTGTAGTCGG CCCTCACGCAGAAAGATCAA 50.0 50.0 57.7 57.9 703 bp 0.25 47.1
ycf1_p5 GGACGCATGGAGCCTTTTAT ACATACCTGCCGAGTTTGTC 50.0 50.0 58.3 57.9 887 bp 0.25 46.7