Hotspot

ndhI

SSC · 121497-122024 · 528 bp

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Pi 0.0647
MarkerSeek score 82.80
Primer available yes
Label ndhI

Feature Metadata

label_name
ndhI
feature_type
gene
region
SSC
length_bp
528
variable_sites
67
pi
0.0647
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI_p1 AAATAAGGAGTTGTGCGGCA ATATGGTCTTTGGGGGTGGA 45.0 50.0 58.1 58.0 1693 bp 0.25 49.2
ndhI_p2 AAATAAGGAGTTGTGCGGCA GGTCTTTGGGGGTGGAATTT 45.0 50.0 58.1 58.0 1689 bp 0.25 49.0
ndhI_p3 AAATAAGGAGTTGTGCGGCA GAATTTGTGGCGTCAACCTG 45.0 50.0 58.1 57.9 1675 bp 0.25 48.8
ndhI_p4 AAATAAGGAGTTGTGCGGCA GGAGCCATATGAGGTGCAAA 45.0 50.0 58.1 57.9 1816 bp 0.25 48.8
ndhI_p5 TATTGATGACCCGACCAGGA ATATGGTCTTTGGGGGTGGA 50.0 50.0 57.8 58.0 1451 bp 0.25 48.6