Hotspot

rpl20-rps12

LSC · 70514-71288 · 775 bp

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Pi 0.0981
MarkerSeek score 83.60
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
775
variable_sites
143
pi
0.0981
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 GACTGCTAGAACTGCTGGTC TATGTCAGCAACAGAAGCCC 55.0 50.0 58.0 57.9 1806 bp 0.25 49.1
rpl20-rps12_p2 GACTGCTAGAACTGCTGGTC CCGAGGAACATGTATTCGGG 55.0 55.0 58.0 58.1 1458 bp 0.25 48.9
rpl20-rps12_p3 GACTGCTAGAACTGCTGGTC TTGTTGATCGTGTAGCGGTT 55.0 45.0 58.0 57.8 1774 bp 0.25 48.7
rpl20-rps12_p4 GACTGCTAGAACTGCTGGTC GGCAAACCCTTATGGATGGT 55.0 50.0 58.0 57.8 1853 bp 0.25 48.7
rpl20-rps12_p5 GACTGCTAGAACTGCTGGTC ATTTGTCCTGGAAGCGGAAG 55.0 50.0 58.0 58.2 1923 bp 0.25 48.6