Hotspot

rpoB-trnC-GCA

LSC · 27356-28362 · 1007 bp

Back to Buxus

Pi 0.128
MarkerSeek score 82.40
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1007
variable_sites
234
pi
0.128
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.92
markerseek_score
82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CGAGCCCATATCCTTGCTTT GCTTTTGTTGATCAGGCGAC 50.0 50.0 58.0 58.0 1603-1680 bp 1.0 91.5
rpoB-trnC-GCA_p2 AGAGGTTCCCAGGGAATTCA GCTTTTGTTGATCAGGCGAC 50.0 50.0 57.9 58.0 1430-1507 bp 1.0 91.3
rpoB-trnC-GCA_p3 CGAGCCCATATCCTTGCTTT TGAATGCCCCAGTACTCAGA 50.0 50.0 58.0 58.0 1954 bp 0.25 49.4
rpoB-trnC-GCA_p4 ATTCCGTTATGGTCCGCTTC GCTTTTGTTGATCAGGCGAC 50.0 50.0 58.1 58.0 1519 bp 0.25 49.3
rpoB-trnC-GCA_p5 CGAGCCCATATCCTTGCTTT GACAAAGTACCTTACGCCGT 50.0 50.0 58.0 57.9 1916 bp 0.25 49.2