Hotspot

rps16-trnQ-UUG

LSC · 5255-6270 · 1016 bp

Back to Buxus

Pi 0.196
MarkerSeek score 79.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1016
variable_sites
331
pi
0.196
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.89
markerseek_score
79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TTTCCTTCTCTTCGCGATCG CGAAAACTGACAGCAGCTTG 50.0 50.0 58.1 58.0 1728 bp 0.25 49.0
rps16-trnQ-UUG_p2 TTTCCTTCTCTTCGCGATCG TCGAAAACTGACAGCAGCTT 50.0 45.0 58.1 58.0 1729 bp 0.25 48.9
rps16-trnQ-UUG_p3 TTTCCTTCTCTTCGCGATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 1132 bp 0.25 48.7
rps16-trnQ-UUG_p4 GGTTTTCCTTCTCTTCGCGA CGAAAACTGACAGCAGCTTG 50.0 50.0 58.2 58.0 1731 bp 0.25 48.1
rps16-trnQ-UUG_p5 GGTTTTCCTTCTCTTCGCGA TCGAAAACTGACAGCAGCTT 50.0 45.0 58.2 58.0 1732 bp 0.25 48.0