Hotspot

matK-trnK-UUU

LSC · 3601-4329 · 729 bp

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Pi 0.104
MarkerSeek score 82.70
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
729
variable_sites
145
pi
0.104
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 CATCTTTCACCCAGCATCGA AACGGTAGAGTACTCGGCTT 50.0 50.0 58.0 58.2 1267 bp 0.25 48.4
matK-trnK-UUU_p2 CATCTTTCACCCAGCATCGA ACGGTAGAGTACTCGGCTTT 50.0 50.0 58.0 58.2 1266 bp 0.25 48.4
matK-trnK-UUU_p3 CATCTTTCACCCAGCATCGA GGGAGGGTTGCTAACTCAAC 50.0 55.0 58.0 58.2 1284 bp 0.25 48.4
matK-trnK-UUU_p4 CCCAGCATCGAAGGATTTGA AACGGTAGAGTACTCGGCTT 50.0 50.0 58.0 58.2 1258 bp 0.25 48.3
matK-trnK-UUU_p5 CCCAGCATCGAAGGATTTGA ACGGTAGAGTACTCGGCTTT 50.0 50.0 58.0 58.2 1257 bp 0.25 48.3