Hotspot

ycf1

SSC · 123654-129167 · 5514 bp

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Pi 0.00464
MarkerSeek score 57.20
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5514
variable_sites
25
pi
0.00464
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GGCACAAATTCTGGGAGGAA TGCTACACATAAAAATCTGGA 50.0 33.3 58.1 52.8 240-309 bp 1.0 43.5
ycf1_p2 AGTGGCACAAATTCTGGGAG TGCTACACATAAAAATCTGGA 50.0 33.3 58.1 52.8 243-312 bp 1.0 43.5
ycf1_p3 AGAGTGGCACAAATTCTGGG TGCTACACATAAAAATCTGGA 50.0 33.3 58.1 52.8 245-314 bp 1.0 43.4
ycf1_p4 GAGTGGCACAAATTCTGGGA TGCTACACATAAAAATCTGGA 50.0 33.3 58.1 52.8 244-313 bp 1.0 43.4
ycf1_p5 ATGCATCAAAGAGTGGCACA TGCTACACATAAAAATCTGGA 45.0 33.3 58.1 52.8 254-323 bp 1.0 43.3