Hotspot

trnP-UGG-psaJ

LSC · 66049-66426 · 378 bp

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Pi 0.00796
MarkerSeek score 55.70
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
378
variable_sites
3
pi
0.00796
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC CCTGCTAAAGACCCAAACCA 50.0 50.0 58.1 57.7 533-534 bp 1.0 77.5
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA CCTGCTAAAGACCCAAACCA 50.0 50.0 58.1 57.7 532-533 bp 1.0 77.5
trnP-UGG-psaJ_p3 ACCCGTGACATTTTGTACCC ACCTGCTAAAGACCCAAACC 50.0 50.0 58.1 57.7 534-535 bp 1.0 77.5
trnP-UGG-psaJ_p4 CCCGTGACATTTTGTACCCA ACCTGCTAAAGACCCAAACC 50.0 50.0 58.1 57.7 533-534 bp 1.0 77.5
trnP-UGG-psaJ_p5 ACAGGATTTGAACCCGTGAC CCTGCTAAAGACCCAAACCA 50.0 50.0 58.1 57.7 544-545 bp 1.0 77.4