Hotspot

rbcL-accD

LSC · 55904-56695 · 792 bp

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Pi 0.00254
MarkerSeek score 60.90
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
792
variable_sites
2
pi
0.00254
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GCAAATGGAGCCCTGAACTA GGGTTCTTCACTTCCACTGG 50.0 55.0 57.9 58.1 1005-1010 bp 1.0 78.8
rbcL-accD_p2 TAGCAAATGGAGCCCTGAAC GGGTTCTTCACTTCCACTGG 50.0 55.0 57.9 58.1 1007-1012 bp 1.0 78.8
rbcL-accD_p3 TGAAGCTAGCAAATGGAGCC GGGTTCTTCACTTCCACTGG 50.0 55.0 58.2 58.1 1013-1018 bp 1.0 77.9
rbcL-accD_p4 CCGTGAAGCTAGCAAATGGA GGGTTCTTCACTTCCACTGG 50.0 55.0 58.3 58.1 1016-1021 bp 1.0 77.7
rbcL-accD_p5 TCCGTGAAGCTAGCAAATGG GGGTTCTTCACTTCCACTGG 50.0 55.0 58.3 58.1 1017-1022 bp 1.0 77.7