Hotspot

petN-psbM

LSC · 28672-29069 · 398 bp

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Pi 0.00806
MarkerSeek score 63.50
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
398
variable_sites
3
pi
0.00806
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 GTCTCACTTGGGCTGCTTTA TGCTACTGCACTGTTCATTCT 50.0 42.9 57.8 57.6 537-554 bp 1.0 71.5
petN-psbM_p2 ACTTGGGCTGCTTTAATGGT TGCTACTGCACTGTTCATTCT 45.0 42.9 57.7 57.6 532-549 bp 1.0 70.6
petN-psbM_p3 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.6 57.6 482-499 bp 1.0 70.4
petN-psbM_p4 GTAAGTCTCACTTGGGCTGC TGCTACTGCACTGTTCATTCT 55.0 42.9 58.6 57.6 541-558 bp 1.0 68.5
petN-psbM_p5 TAAGTCTCACTTGGGCTGCT TGCTACTGCACTGTTCATTCT 50.0 42.9 58.6 57.6 540-557 bp 1.0 67.8