Hotspot

atpH-atpI

LSC · 14164-14980 · 817 bp

Pi 0.00491

MarkerSeek score 61.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 4
pi: 0.00491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACGTTAGCCGC	45.0	45.0	58.0	58.6	904-906 bp	1.0	75.5
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.0	57.4	876-878 bp	1.0	75.5
atpH-atpI_p3	TACCTTCTACAGCTTGGCCT	ATTTTTGCAACGTTAGCCGC	50.0	45.0	58.0	58.6	987-989 bp	1.0	75.4
atpH-atpI_p4	TACCTTCTACAGCTTGGCCT	TAGGTGAATCCATGGAGGGT	50.0	50.0	58.0	57.4	959-961 bp	1.0	75.4
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	ATTTTTGCAACGTTAGCCGC	50.0	45.0	57.9	58.6	960-962 bp	1.0	74.9