Hotspot

trnS-GCU-trnG-UCC

LSC · 8273-9352 · 1080 bp

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Pi 0.0056
MarkerSeek score 62.70
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1080
variable_sites
6
pi
0.0056
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA ACGAATCACACTTTTACCACT 45.0 38.1 58.0 55.5 1154-1193 bp 1.0 52.4
trnS-GCU-trnG-UCC_p2 ATTAGCAATCCGCCGCTTTA AGAACGAATCACACTTTTACCAC 45.0 39.1 58.0 57.3 1157-1196 bp 1.0 51.3
trnS-GCU-trnG-UCC_p3 ATTAGCAATCCGCCGCTTTA GAACGAATCACACTTTTACCACT 45.0 39.1 58.0 57.3 1156-1195 bp 1.0 51.3
trnS-GCU-trnG-UCC_p4 AGAAGAAGCGGAGAGAGAGG ACGAATCACACTTTTACCACT 55.0 38.1 58.2 55.5 1210-1249 bp 1.0 50.7
trnS-GCU-trnG-UCC_p5 ATTAGCAATCCGCCGCTTTA AACGAATCACACTTTTACCACT 45.0 36.4 58.0 56.2 1155-1194 bp 1.0 50.4