Hotspot

ycf1-trnN-GUU

SSC · 130517-134338 · 3822 bp

Back to Bursera

Pi 0.00835

MarkerSeek score 50.60

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: SSC
length_bp: 3822
variable_sites: 60
pi: 0.00835
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	ATTGTTTGAGCGTTGGGAGT	TCACCTATAATCCTAAATGAAACTTCT	45.0	29.6	58.0	56.4	170 bp	1.0	43.8
ycf1-trnN-GUU_p2	ATTGTTTGAGCGTTGGGAGT	TCACCTATAATCCTAAATGAAACT	45.0	29.2	58.0	53.2	170 bp	1.0	43.8
ycf1-trnN-GUU_p3	TGTTTGAGCGTTGGGAGTAG	TCACCTATAATCCTAAATGAAACTTCT	50.0	29.6	57.8	56.4	168 bp	1.0	43.8
ycf1-trnN-GUU_p4	ATTGTTTGAGCGTTGGGAGT	TCACCTATAATCCTAAATGAAACTTC	45.0	30.8	58.0	55.1	170 bp	1.0	43.8
ycf1-trnN-GUU_p5	ATTGTTTGAGCGTTGGGAGT	CACCTATAATCCTAAATGAAACTTCT	45.0	30.8	58.0	55.1	169 bp	1.0	43.8