Hotspot

ccsA-ndhD

SSC · 120038-120363 · 326 bp

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Pi 0.0374

MarkerSeek score 68.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 326
variable_sites: 20
pi: 0.0374
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGTGGCTTCTATGGGCTTTC	AGTGGACAAGGTCGAAGCTA	50.0	50.0	57.9	58.4	431-466 bp	1.0	83.5
ccsA-ndhD_p2	TTCCGCAATTGTGGCTTCTA	AGTGGACAAGGTCGAAGCTA	45.0	50.0	57.8	58.4	440-475 bp	1.0	82.9
ccsA-ndhD_p3	AATTCCGCAATTGTGGCTTC	AGTGGACAAGGTCGAAGCTA	45.0	50.0	57.6	58.4	442-477 bp	1.0	81.5
ccsA-ndhD_p4	TGTGGCTTCTATGGGCTTTC	TCCGGATTTCGTTCTCTCAC	50.0	50.0	57.9	57.4	455-490 bp	1.0	81.1
ccsA-ndhD_p5	TGTGGCTTCTATGGGCTTTC	CCGGATTTCGTTCTCTCACT	50.0	50.0	57.9	57.3	454-489 bp	1.0	81.0