Hotspot

psbB-psbT

LSC · 77738-77935 · 198 bp

Back to Bursera

Pi 0.00909

MarkerSeek score 58.20

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 198
variable_sites: 4
pi: 0.00909
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GTTCCGGGATGTTTTTGCTG	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.9	57.6	385-394 bp	1.0	71.0
psbB-psbT_p2	GAACCTTGTTCCGGGATGTT	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	58.1	57.6	392-401 bp	1.0	70.7
psbB-psbT_p3	TGTTCCGGGATGTTTTTGCT	TGGAACTTTAGGTGGTTCTCG	45.0	47.6	58.3	57.6	386-395 bp	1.0	69.1
psbB-psbT_p4	GTTCCGGGATGTTTTTGCTG	GGAACTTTAGGTGGTTCTCGA	50.0	47.6	57.9	57.3	384-393 bp	1.0	68.8
psbB-psbT_p5	GAACCTTGTTCCGGGATGTT	GGAACTTTAGGTGGTTCTCGA	50.0	47.6	58.1	57.3	391-400 bp	1.0	68.5