Hotspot

psbE-petL

LSC · 68005-69447 · 1443 bp

Back to Bursera

Pi 0.00721

MarkerSeek score 56.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1443
variable_sites: 21
pi: 0.00721
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.9	56.3	1407-1591 bp	1.0	71.0
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1330-1514 bp	1.0	70.2
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.3	1416-1600 bp	1.0	70.0
psbE-petL_p4	TGCTGACGAATAACCAACCC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	58.2	56.3	1421-1605 bp	1.0	69.9
psbE-petL_p5	CGAATAACCAACCCGCAATG	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1415-1599 bp	1.0	69.9