Hotspot

petA-psbJ

LSC · 66089-67218 · 1130 bp

Back to Bursera

Pi 0.00973

MarkerSeek score 60.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1130
variable_sites: 23
pi: 0.00973
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	TATTCCGGATTGGGTTCGTC	50.0	50.0	58.0	57.4	1079-1277 bp	1.0	80.4
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	TATTCCGGATTGGGTTCGTC	50.0	50.0	58.2	57.4	1076-1274 bp	1.0	79.2
petA-psbJ_p3	ATCCATTACGTGTCCAAGGC	GGATTGGGTTCGTCCTTGTA	50.0	50.0	58.0	57.2	1073-1271 bp	1.0	79.0
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	GGATTGGGTTCGTCCTTGTA	50.0	50.0	58.2	57.2	1070-1268 bp	1.0	77.9
petA-psbJ_p5	TTACGTGTCCAAGGCCTTTT	TATTCCGGATTGGGTTCGTC	45.0	50.0	57.6	57.4	1074-1272 bp	1.0	77.9