Hotspot

trnF-GAA-ndhJ

LSC · 51083-51759 · 677 bp

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Pi 0.0194

MarkerSeek score 62.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 677
variable_sites: 24
pi: 0.0194
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGGCTGAAACGTATC	55.0	50.0	58.0	58.1	834-851 bp	1.0	83.3
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	802-819 bp	1.0	83.3
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATCCACGGCTGAAACGTATC	50.0	50.0	58.1	58.1	798-815 bp	1.0	83.1
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	766-783 bp	1.0	83.1
trnF-GAA-ndhJ_p5	ATGATGCGTCGGTAATGGTC	ATCCACGGCTGAAACGTATC	50.0	50.0	58.1	58.1	854-871 bp	1.0	82.2