Hotspot

trnG-GCC-trnfM-CAU

LSC · 38654-38833 · 180 bp

Back to Bursera

Pi 0.0267

MarkerSeek score 65.10

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 10
pi: 0.0267
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	258-262 bp	1.0	81.4
trnG-GCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	CTTTTCTTCATCGCGGGGTA	50.0	50.0	57.8	58.0	309-313 bp	1.0	81.1
trnG-GCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	TTTTCTTCATCGCGGGGTAG	50.0	50.0	57.8	58.0	308-312 bp	1.0	81.1
trnG-GCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	294-298 bp	1.0	81.1
trnG-GCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	256-260 bp	1.0	80.3