Hotspot

psbZ-trnG-GCC

LSC · 38135-38582 · 448 bp

Back to Bursera

Pi 0.0125

MarkerSeek score 56.00

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 448
variable_sites: 12
pi: 0.0125
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	606-621 bp	1.0	78.1
psbZ-trnG-GCC_p2	TGAGGGTTGGTCGAGTAACA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	595-610 bp	1.0	78.0
psbZ-trnG-GCC_p3	CTGAGGGTTGGTCGAGTAAC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	57.6	57.8	596-611 bp	1.0	77.4
psbZ-trnG-GCC_p4	CCTGAGGGTTGGTCGAGTAA	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.7	57.8	597-612 bp	1.0	74.5
psbZ-trnG-GCC_p5	GAGGGTTGGTCGAGTAACAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.2	57.8	594-609 bp	1.0	73.8