Hotspot

trnD-GUC-trnY-GUA

LSC · 31745-32235 · 491 bp

Back to Bursera

Pi 0.00672

MarkerSeek score 58.00

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 491
variable_sites: 6
pi: 0.00672
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	TTTTCTTCCTGGGTCGATGC	50.0	50.0	58.5	58.2	513-615 bp	1.0	79.6
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	459-561 bp	1.0	79.2
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	458-560 bp	1.0	79.2
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	480-582 bp	1.0	78.2
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	479-581 bp	1.0	78.0