Hotspot

psbM-trnD-GUC

LSC · 31138-31670 · 533 bp

Back to Bursera

Pi 0.0122

MarkerSeek score 55.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 533
variable_sites: 13
pi: 0.0122
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TCCCCGGGATTGTAGTTCAA	45.0	50.0	55.8	58.3	658-665 bp	1.0	61.1
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TTCCCCGGGATTGTAGTTCA	45.0	50.0	55.8	58.3	659-666 bp	1.0	61.1
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	643-650 bp	1.0	60.1
psbM-trnD-GUC_p4	AGAATGAACAGTGCAGTAGC	TTTGATTTCCCCGGGATTGT	45.0	45.0	55.8	57.4	665-672 bp	1.0	58.9
psbM-trnD-GUC_p5	AGGAACTAGAATGAACAGTGCA	TCCCCGGGATTGTAGTTCAA	40.9	50.0	57.4	58.3	665-672 bp	1.0	58.0