Hotspot

ycf1

IRb · 107871-115106 · 7236 bp

Back to Burmeistera

Pi 0.00775

MarkerSeek score 50.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 7236
variable_sites: 220
pi: 0.00775
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCAGAGCGAATTCGAATGA	CGAAATCTACTAGCCTCGGC	50.0	55.0	58.1	57.9	442-661 bp	1.0	87.6
ycf1_p2	CCCAGAGCGAATTCGAATGA	CATCAAACCAAATTCCGCCC	50.0	50.0	58.1	58.0	675-894 bp	1.0	87.1
ycf1_p3	CCCAGAGCGAATTCGAATGA	CGCCCAGCTAAACAGAGTAG	50.0	55.0	58.1	58.1	660-879 bp	1.0	86.7
ycf1_p4	CCCAGAGCGAATTCGAATGA	CGGCAGATCTCCCATTTTCA	50.0	50.0	58.1	58.0	606-825 bp	0.941176	84.1
ycf1_p5	CCCAGAGCGAATTCGAATGA	CCCACCATCTGCATTAAGCT	50.0	50.0	58.1	57.9	626-758 bp	0.882353	82.3