Hotspot

rpl32-ndhF

SSC · 125363-126174 · 812 bp

Back to Burmeistera

Pi 0.0103

MarkerSeek score 64.60

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 812
variable_sites: 53
pi: 0.0103
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CGCCGATGAATATCCCTTCC	AGGAACTGAAAGGGGAAGGA	55.0	50.0	58.3	57.9	880-996 bp	1.0	85.2
rpl32-ndhF_p2	CGCCGATGAATATCCCTTCC	GGAACTGAAAGGGGAAGGAA	55.0	50.0	58.3	57.0	879-995 bp	1.0	78.5
rpl32-ndhF_p3	CGCCGATGAATATCCCTTCC	ACTGAAAGGGGAAGGAAAGG	55.0	50.0	58.3	57.0	876-992 bp	1.0	78.4
rpl32-ndhF_p4	CGCCGATGAATATCCCTTCC	CTGAAAGGGGAAGGAAAGGT	55.0	50.0	58.3	57.0	875-991 bp	1.0	78.4
rpl32-ndhF_p5	GCCGATGAATATCCCTTCCT	AGGAACTGAAAGGGGAAGGA	50.0	50.0	56.8	57.9	879-995 bp	1.0	78.1