Hotspot

trnL (UAG)-rpl32

SSC · 124737-125200 · 464 bp

Back to Burmeistera

Pi 0.0132
MarkerSeek score 59.80
Primer available yes
Label trnL (UAG)-rpl32

Feature Metadata

label_name
trnL (UAG)-rpl32
feature_type
igs
region
SSC
length_bp
464
variable_sites
35
pi
0.0132
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL (UAG)-rpl32_p1 AGCGTGTCTACCGATTTCAC GGAAGGGATATTCATCGGCG 50.0 55.0 58.0 58.3 580-592 bp 1.0 85.0
trnL (UAG)-rpl32_p2 CAGCGTGTCTACCGATTTCA GGAAGGGATATTCATCGGCG 50.0 55.0 58.0 58.3 581-593 bp 1.0 85.0
trnL (UAG)-rpl32_p3 AGCGTGTCTACCGATTTCAC ACACTGAGTCACCTCTTCGT 50.0 50.0 58.0 58.3 670-682 bp 1.0 83.8
trnL (UAG)-rpl32_p4 CAGCGTGTCTACCGATTTCA ACACTGAGTCACCTCTTCGT 50.0 50.0 58.0 58.3 671-683 bp 1.0 83.7
trnL (UAG)-rpl32_p5 GGTGTTTTGAAGACGGCATG GGAAGGGATATTCATCGGCG 50.0 55.0 57.9 58.3 649-661 bp 0.941176 81.7