Hotspot

petA-psbJ

LSC · 62168-63140 · 973 bp

Back to Burmeistera

Pi 0.0177

MarkerSeek score 70.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 973
variable_sites: 65
pi: 0.0177
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGCAGTTCGAGAAGGTTCAA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.1	57.6	985-1047 bp	1.0	78.9
petA-psbJ_p2	AAGCAGTTCGAGAAGGTTCA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.1	57.6	986-1048 bp	1.0	78.8
petA-psbJ_p3	AGAAGCAGTTCGAGAAGGTTC	ATTCCGGATTGGGTTCATCC	47.6	50.0	58.0	57.6	988-1050 bp	1.0	77.9
petA-psbJ_p4	CGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	50.0	50.0	56.8	57.6	978-1040 bp	1.0	76.9
petA-psbJ_p5	AAGCAGTTCGAGAAGGTTCAA	ATTCCGGATTGGGTTCATCC	42.9	50.0	57.8	57.6	986-1048 bp	1.0	76.6