Hotspot

rpoB-trnC (GCA)

LSC · 33131-35078 · 1948 bp

Back to Burmeistera

Pi 0.01

MarkerSeek score 58.20

Primer available yes

Label rpoB-trnC (GCA)

Feature Metadata

label_name: rpoB-trnC (GCA)
feature_type: igs
region: LSC
length_bp: 1948
variable_sites: 102
pi: 0.01
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC (GCA)_p1	CCTGATCGATGAACCTGCAA	GGTGCAGAGTCAAAAAGGGA	50.0	50.0	58.0	58.0	2299-2406 bp	1.0	85.8
rpoB-trnC (GCA)_p2	AGTCCCGCGGATACATAGAA	GGTGCAGAGTCAAAAAGGGA	50.0	50.0	58.0	58.0	2468-2575 bp	1.0	85.7
rpoB-trnC (GCA)_p3	TAGACTCGACCCCATAGCTG	GGTGCAGAGTCAAAAAGGGA	55.0	50.0	58.0	58.0	2784-2891 bp	1.0	85.3
rpoB-trnC (GCA)_p4	TCGACCCCATAGCTGATGAT	GGTGCAGAGTCAAAAAGGGA	50.0	50.0	58.0	58.0	2779-2886 bp	0.941176	82.7
rpoB-trnC (GCA)_p5	ATTGGATCCCCACCTACACA	GGTGCAGAGTCAAAAAGGGA	50.0	50.0	58.0	58.0	2924-2993 bp	0.764706	74.8