Hotspot

trnQ (UUG)-psbK

LSC · 12991-13709 · 719 bp

Back to Burmeistera

Pi 0.0597

MarkerSeek score 78.90

Primer available yes

Label trnQ (UUG)-psbK

Feature Metadata

label_name: trnQ (UUG)-psbK
feature_type: igs
region: LSC
length_bp: 719
variable_sites: 141
pi: 0.0597
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ (UUG)-psbK_p1	TTGGAAGTGAGCCACCTTTC	CCAGGTCCGTGGAAAGAAAA	50.0	50.0	58.0	58.0	2290-2977 bp	0.882353	86.9
trnQ (UUG)-psbK_p2	TTGGAAGTGAGCCACCTTTC	CCAAGATGACTTCATGCCGA	50.0	50.0	58.0	58.0	1566-2253 bp	0.882353	86.9
trnQ (UUG)-psbK_p3	TGCTTGTAGTCTTTCGGGTG	CCAAGATGACTTCATGCCGA	50.0	50.0	57.8	58.0	1340-1824 bp	0.823529	83.4
trnQ (UUG)-psbK_p4	CGGCAATACCCATAGCACAT	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.1	57.9	784-1153 bp	0.411765	63.9
trnQ (UUG)-psbK_p5	CGGCAATACCCATAGCACAT	CCAAGATGACTTCATGCCGA	50.0	50.0	58.1	58.0	1063-1432 bp	0.411765	63.7