Hotspot

rps16-trnQ (UUG)

LSC · 11604-12918 · 1315 bp

Back to Burmeistera

Pi 0.15

MarkerSeek score 76.50

Primer available yes

Label rps16-trnQ (UUG)

Feature Metadata

label_name: rps16-trnQ (UUG)
feature_type: igs
region: LSC
length_bp: 1315
variable_sites: 506
pi: 0.15
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ (UUG)_p1	AGCGAACATCAATTGCAACG	CCAAGATGACTTCATGCCGA	45.0	50.0	58.0	58.0	1953-2637 bp	1.0	91.5
rps16-trnQ (UUG)_p2	GCGAACATCAATTGCAACGA	CCAAGATGACTTCATGCCGA	45.0	50.0	58.0	58.0	1952-2636 bp	1.0	91.5
rps16-trnQ (UUG)_p3	TTGCTTTCTACCACAGCGTT	CCAAGATGACTTCATGCCGA	45.0	50.0	58.0	58.0	1916-2600 bp	1.0	91.5
rps16-trnQ (UUG)_p4	AGCGAACATCAATTGCAACG	CTTACAGCAGCTTGCCAAAC	45.0	50.0	58.0	57.9	1674-2358 bp	1.0	91.3
rps16-trnQ (UUG)_p5	TGCTTTCTACCACAGCGTTT	CCAAGATGACTTCATGCCGA	45.0	50.0	58.0	58.0	1915-2599 bp	0.882353	86.8