Hotspot

trnK (UUU)-rps16

LSC · 10757-11336 · 580 bp

Back to Burmeistera

Pi 0.0117

MarkerSeek score 60.00

Primer available yes

Label trnK (UUU)-rps16

Feature Metadata

label_name: trnK (UUU)-rps16
feature_type: igs
region: LSC
length_bp: 580
variable_sites: 40
pi: 0.0117
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK (UUU)-rps16_p1	TAAGAATGGGAGCTCGTTGC	GCCCAAAAACCAAAAACCGA	50.0	45.0	58.0	58.0	1387-1452 bp	1.0	90.8
trnK (UUU)-rps16_p2	TAAGAATGGGAGCTCGTTGC	CTCAATCCTCGGAACCTTGG	50.0	55.0	58.0	58.0	1366-1431 bp	1.0	90.8
trnK (UUU)-rps16_p3	TAAGAATGGGAGCTCGTTGC	CGTTGCAATTGATGTTCGCT	50.0	45.0	58.0	58.0	1094-1135 bp	0.941176	85.3
trnK (UUU)-rps16_p4	TAAGAATGGGAGCTCGTTGC	CGCACTCTTCCAATAAGGCT	50.0	50.0	58.0	58.0	1569-1628 bp	0.470588	64.5
trnK (UUU)-rps16_p5	TAAGAATGGGAGCTCGTTGC	GAAAGGTGGCTCACTTCCAA	50.0	50.0	58.0	58.0	1477-1525 bp	0.294118	56.5