Hotspot

ycf1

SSC · 109625-115096 · 5472 bp

Back to Burmannia

Pi 0.0617

MarkerSeek score 69.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5472
variable_sites: 371
pi: 0.0617
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAAAGCCCACATACGTCGAA	TTGCTTATTTCATTGCGCGT	45.0	40.0	57.8	57.4	747 bp	0.333333	46.9
ycf1_p2	CTAAACTGCCCGTTCTCCAA	TTGCTTATTTCATTGCGCGT	50.0	40.0	57.8	57.4	1231 bp	0.333333	46.9
ycf1_p3	AAAGCCCACATACGTCGAAT	TTGCTTATTTCATTGCGCGT	45.0	40.0	57.6	57.4	746 bp	0.333333	45.1
ycf1_p4	TTCTAAACTGCCCGTTCTCC	TTGCTTATTTCATTGCGCGT	50.0	40.0	57.5	57.4	1233 bp	0.333333	44.6
ycf1_p5	CAAAAGCCCACATACGTCGA	TTGCTTATTTCATTGCGCGT	50.0	40.0	58.6	57.4	748 bp	0.333333	43.8