Hotspot

psaB

LSC · 35531-37735 · 2205 bp

Back to Burmannia

Pi 0.0602

MarkerSeek score 81.40

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: LSC
length_bp: 2205
variable_sites: 196
pi: 0.0602
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	TTTCTGCCGTTTCTTCTCCC	TGCTCATTTTGTCTGGGCTT	50.0	45.0	58.1	58.0	2649-2654 bp	0.666667	67.6
psaB_p2	AACAACGACGATGAAGACGT	TGCTCATTTTGTCTGGGCTT	45.0	45.0	57.9	58.0	2794-2799 bp	0.666667	67.5
psaB_p3	TTTCTGCCGTTTCTTCTCCC	CGGGATTTCTTATGGGCACA	50.0	50.0	58.1	57.9	2732-2737 bp	0.666667	67.1
psaB_p4	TTTCTGCCGTTTCTTCTCCC	ACGGGATTTCTTATGGGCAC	50.0	50.0	58.1	57.9	2733-2738 bp	0.666667	67.1
psaB_p5	GCCCCTCCCTTACCATATCT	TTTAGTGGCCGGGGTTATTG	55.0	50.0	58.0	57.9	2996 bp	0.333333	51.9