Hotspot

ndhI

SSC · 119336-119818 · 483 bp

Back to Burmannia

Pi 0.0697

MarkerSeek score 82.60

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 483
variable_sites: 49
pi: 0.0697
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	AGTGGGGACTAACGTTAACG	GGAGCCGTATGAAACTGAGG	50.0	55.0	57.3	58.1	1462 bp	0.333333	47.0
ndhI_p2	AGTGGGGACTAACGTTAACG	TACCAGAAGCGGAAGAGGAA	50.0	50.0	57.3	58.1	1241 bp	0.333333	47.0
ndhI_p3	AGTGGGGACTAACGTTAACG	TTACCAGAAGCGGAAGAGGA	50.0	50.0	57.3	58.1	1242 bp	0.333333	47.0
ndhI_p4	AGTGGGGACTAACGTTAACG	GGCGTCAACCTGTAGGATTT	50.0	50.0	57.3	57.9	1313 bp	0.333333	46.6
ndhI_p5	AGTGGGGACTAACGTTAACG	TGTAACCATAGAGGAGCCGT	50.0	50.0	57.3	57.8	1474 bp	0.333333	46.3