Hotspot

psbE-petL

LSC · 63489-64638 · 1150 bp

Back to Burmannia

Pi 0.107

MarkerSeek score 68.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1150
variable_sites: 71
pi: 0.107
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.46
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	GAACGTTCTCCCGTACTTCC	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.0	58.0	1114-1728 bp	0.666667	75.2
psbE-petL_p2	TCACAAATAACCAACCCGCA	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1203-1817 bp	0.666667	74.9
psbE-petL_p3	GAACGTTCTCCCGTACTTCC	TTAACTGATCACCGCGTCTG	55.0	50.0	58.0	58.0	1554 bp	0.333333	53.2
psbE-petL_p4	TCACAAATAACCAACCCGCA	TTAACTGATCACCGCGTCTG	45.0	50.0	58.0	58.0	1643 bp	0.333333	53.1
psbE-petL_p5	AAAGAGTCAAAACGCCCAGT	TTAACTGATCACCGCGTCTG	45.0	50.0	57.9	58.0	1746 bp	0.333333	52.8