Hotspot

ycf4-cemA

LSC · 59251-60151 · 901 bp

Back to Burmannia

Pi 0.116

MarkerSeek score 78.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 901
variable_sites: 82
pi: 0.116
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GCGTGTGCCAATGGAAGTAT	GCAAGATAGTATAGGAAAGTCAATGC	50.0	38.5	58.9	58.2	792-969 bp	0.666667	38.1
ycf4-cemA_p2	TTTTTGCGTGTGCCAATGG	GCAAGATAGTATAGGAAAGTCAATGC	47.4	38.5	58.0	58.2	797-974 bp	0.666667	38.1
ycf4-cemA_p3	GCGTGTGCCAATGGAAGTAT	TGCAAGATAGTATAGGAAAGTCA	50.0	34.8	58.9	54.6	793-970 bp	0.666667	38.1
ycf4-cemA_p4	TTTTTGCGTGTGCCAATGG	TGCAAGATAGTATAGGAAAGTCA	47.4	34.8	58.0	54.6	798-975 bp	0.666667	38.1
ycf4-cemA_p5	GCGTGTGCCAATGGAAGTAT	TGCAAGATAGTATAGGAAAGTCAA	50.0	33.3	58.9	55.4	793-970 bp	0.666667	38.1