Hotspot

atpB-rbcL

LSC · 52519-53379 · 861 bp

Back to Burmannia

Pi 0.0808

MarkerSeek score 81.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 861
variable_sites: 93
pi: 0.0808
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AATAGCCCGATTTTTCCCCC	GGTAGCATCGCCCTTTGTAA	50.0	50.0	57.9	58.0	1604-1626 bp	0.666667	71.4
atpB-rbcL_p2	AATAGCCCGATTTTTCCCCC	CGGTCTCTCCAACGCATAAA	50.0	50.0	57.9	58.0	1998-2020 bp	0.666667	71.0
atpB-rbcL_p3	CTGTTTTACCTACCCCAGCC	CGGTCTCTCCAACGCATAAA	55.0	50.0	57.9	58.0	2024-2046 bp	0.666667	70.5
atpB-rbcL_p4	CGGCGGTTCATTCATTTGAC	CGGTCTCTCCAACGCATAAA	50.0	50.0	58.1	58.0	2215-2237 bp	0.666667	70.4
atpB-rbcL_p5	GCGGTTCATTCATTTGACCG	CGGTCTCTCCAACGCATAAA	50.0	50.0	58.1	58.0	2213-2235 bp	0.666667	70.4