Hotspot

trnF-GAA-ndhJ

LSC · 46739-47226 · 488 bp

Back to Burmannia

Pi 0.0975

MarkerSeek score 81.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 488
variable_sites: 64
pi: 0.0975
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAATACAAGATGCTCATTGA	50.0	31.8	58.1	53.8	532-546 bp	1.0	52.0
trnF-GAA-ndhJ_p2	AATCCTCGTGTCACCAGTTC	TGAAATACAAGATGCTCATTGA	50.0	31.8	57.5	53.8	535-549 bp	1.0	52.0
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATGAAATACAAGATGCTCATTGA	50.0	30.4	58.1	54.4	533-547 bp	1.0	52.0
trnF-GAA-ndhJ_p4	TGGTAGAGCAGAGGACTGAA	TGAAATACAAGATGCTCATTGA	50.0	31.8	57.4	53.8	555-569 bp	1.0	52.0
trnF-GAA-ndhJ_p5	TTGGTAGAGCAGAGGACTGA	TGAAATACAAGATGCTCATTGA	50.0	31.8	57.4	53.8	556-570 bp	1.0	52.0