Hotspot

psbM-trnD-GUC

LSC · 27950-28595 · 646 bp

Back to Burmannia

Pi 0.126

MarkerSeek score 80.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 646
variable_sites: 81
pi: 0.126
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TTGACTCACTGTTTTCACGT	GTTCGAGCCCCGTCAATC	40.0	61.1	55.5	58.2	558-763 bp	0.666667	37.9
psbM-trnD-GUC_p2	TGACTCACTGTTTTCACGTAGA	GTTCGAGCCCCGTCAATC	40.9	61.1	57.4	58.2	557-762 bp	0.666667	37.0
psbM-trnD-GUC_p3	TTTGACTCACTGTTTTCACGT	GTTCGAGCCCCGTCAATC	38.1	61.1	56.3	58.2	559-764 bp	0.666667	36.2
psbM-trnD-GUC_p4	TTGACTCACTGTTTTCACGTAGA	GTTCGAGCCCCGTCAATC	39.1	61.1	58.0	58.2	558-763 bp	0.666667	35.8
psbM-trnD-GUC_p5	TTTTGACTCACTGTTTTCACGT	GTTCGAGCCCCGTCAATC	36.4	61.1	57.0	58.2	560-765 bp	0.666667	35.7