Hotspot

ycf1

SSC · 126000-131492 · 5493 bp

Back to Bupleurum

Pi 0.0177

MarkerSeek score 60.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5493
variable_sites: 655
pi: 0.0177
unique_haplotype_count: 42
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACGTTTTTGATAACCCCCTGT	GCACAGATCTAAATCAAGGCC	42.9	47.6	57.8	57.0	270-285 bp	0.906977	61.2
ycf1_p2	ACGTTTTTGATAACCCCCTGT	GGCACAGATCTAAATCAAGGC	42.9	47.6	57.8	57.0	271-286 bp	0.906977	61.2
ycf1_p3	ACGTTTTTGATAACCCCCTGT	GCACAGATCTAAATCAAGGCCT	42.9	45.5	57.8	58.5	270-285 bp	0.906977	57.4
ycf1_p4	ACGTTTTTGATAACCCCCTGT	TGGCACAGATCTAAATCAAGGC	42.9	45.5	57.8	58.7	272-287 bp	0.906977	55.3
ycf1_p5	CGTTTTTGATAACCCCCTGT	GCACAGATCTAAATCAAGGCC	45.0	47.6	55.9	57.0	269-284 bp	0.906977	54.5