Hotspot

ndhC-trnV-UAC

LSC · 51753-52967 · 1215 bp

Back to Bupleurum

Pi 0.0266

MarkerSeek score 73.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1215
variable_sites: 199
pi: 0.0266
unique_haplotype_count: 42
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	31.8	55.0	53.9	58.1	1068-1288 bp	1.0	52.0
ndhC-trnV-UAC_p2	TCATATTCGTAAAGCAGAAACA	GCGTGTTGGGTCTTTGAAAC	31.8	50.0	53.9	58.2	1129-1349 bp	1.0	52.0
ndhC-trnV-UAC_p3	ATCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	30.4	55.0	54.5	58.1	1069-1289 bp	1.0	52.0
ndhC-trnV-UAC_p4	TCATATTCGTAAAGCAGAAACAT	CCGAGAAAGTCTACGGTTCG	30.4	55.0	54.5	58.1	1068-1288 bp	1.0	52.0
ndhC-trnV-UAC_p5	TCATATTCGTAAAGCAGAAACAT	GCGTGTTGGGTCTTTGAAAC	30.4	50.0	54.5	58.2	1129-1349 bp	1.0	52.0