Hotspot

trnE-UUC-trnT-GGU

LSC · 32253-32940 · 688 bp

Back to Bupleurum

Pi 0.0221

MarkerSeek score 71.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 95
pi: 0.0221
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	759-846 bp	1.0	90.6
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	724-811 bp	1.0	89.8
trnE-UUC-trnT-GGU_p3	TTTGTAGTACCCCCTACCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.7	58.0	797-884 bp	1.0	89.8
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	756-843 bp	1.0	89.4
trnE-UUC-trnT-GGU_p5	TTTGTAGTACCCCCTACCCC	TACCGCTGAGTTAAAAGGGC	55.0	50.0	57.7	57.9	762-849 bp	1.0	89.0