Hotspot

rpoB-trnC-GCA

LSC · 27783-29044 · 1262 bp

Back to Bupleurum

Pi 0.0207

MarkerSeek score 73.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1262
variable_sites: 172
pi: 0.0207
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGCCCTGATCCATGAACCTA	CTTACCACTCGGCCATATCG	50.0	55.0	57.8	57.9	1242-1385 bp	1.0	89.9
rpoB-trnC-GCA_p2	AGCCCTGATCCATGAACCTA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1282-1425 bp	1.0	89.8
rpoB-trnC-GCA_p3	GCCCTGATCCATGAACCTAC	CTTACCACTCGGCCATATCG	55.0	55.0	57.7	57.9	1241-1384 bp	1.0	89.4
rpoB-trnC-GCA_p4	TCCCTCATTTCCATCTCGGA	CTTACCACTCGGCCATATCG	50.0	55.0	57.8	57.9	1175-1318 bp	0.976744	89.1
rpoB-trnC-GCA_p5	TCCCTCATTTCCATCTCGGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1215-1358 bp	0.976744	89.0