Hotspot

atpF-atpH

LSC · 13916-14349 · 434 bp

Back to Bupleurum

Pi 0.0487

MarkerSeek score 89.40

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 434
variable_sites: 93
pi: 0.0487
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 89.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.9	58.2	442-579 bp	0.953488	87.2
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	444-581 bp	0.976744	86.8
atpF-atpH_p3	CCAGTGGCCCAAATAAACGA	GCCTGGTTGTAGCATTAGCA	50.0	50.0	58.5	58.2	415-552 bp	0.976744	85.4
atpF-atpH_p4	CCAGTGGCCCAAATAAACGA	TGGCCTGGTTGTAGCATTAG	50.0	50.0	58.5	57.6	417-554 bp	1.0	85.0
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	TTTATGGCCTGGTTGTAGCA	50.0	45.0	57.9	57.1	448-585 bp	0.976744	83.0