Hotspot

trnK-UUU-rps16

LSC · 4410-5287 · 878 bp

Back to Bupleurum

Pi 0.0324

MarkerSeek score 73.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 878
variable_sites: 186
pi: 0.0324
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCCTTTCAGGATCAGTCGTG	CTTTTTCTCGAGCCGTACGA	55.0	50.0	58.0	58.0	1231-1392 bp	1.0	91.7
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	CTTTTTCTCGAGCCGTACGA	55.0	50.0	58.0	58.0	1230-1391 bp	1.0	91.7
trnK-UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	GCCGTACGAGGAGAAAACTT	55.0	50.0	58.0	57.9	1219-1380 bp	0.976744	90.3
trnK-UUU-rps16_p4	CCCTTTCAGGATCAGTCGTG	TGCTGCCATTTTGGAAGGAT	55.0	45.0	58.0	58.1	1613-1774 bp	0.813953	84.0
trnK-UUU-rps16_p5	CCTTTCAGGATCAGTCGTGG	TGCTGCCATTTTGGAAGGAT	55.0	45.0	58.0	58.1	1612-1773 bp	0.813953	84.0