Hotspot

ndhD-psaC

SSC · 116570-116704 · 135 bp

Back to Bunias

Pi 0.0519
MarkerSeek score 75.90
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
135
variable_sites
7
pi
0.0519
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 ACGAACCAGCAGATATTGGA AAACAACTCGAAGCATGGGT 45.0 45.0 56.6 58.0 243-247 bp 1.0 71.2
ndhD-psaC_p2 ACGAACCAGCAGATATTGGA GGCATGAAACAACTCGAAGC 45.0 50.0 56.6 58.0 249-253 bp 1.0 71.0
ndhD-psaC_p3 ACGAACCAGCAGATATTGGA TGTGAATCTGCCTGTCCAAC 45.0 50.0 56.6 58.1 298-302 bp 1.0 70.1
ndhD-psaC_p4 ACGAACCAGCAGATATTGGA AAGAACCGAGGACTGTGTTG 45.0 50.0 56.6 57.8 329-333 bp 1.0 68.8
ndhD-psaC_p5 ACGAACCAGCAGATATTGGA CAAGAACCGAGGACTGTGTT 45.0 50.0 56.6 57.8 330-334 bp 1.0 68.8